THE SECTION FREEZE-SUBSTITUTION TECHNIQUE: I. METHOD
نویسندگان
چکیده
منابع مشابه
Fixing Conditions in the Freeze Substitution Technique for Light Microscopy Observation of Frozen Beef Tissue
The freeze substitution histological technique allows the indirect light microscopicobservation and the quantitative evaluation of ice crystal size in frozen tissues. Theuseofchemical fixatives in substitu ting fluids improves morphological and histochemical preservation of the tissue. Fixation conditions become important since this step can introduce modifications in crystal sizes . Effects of...
متن کاملEpoxy resin as fixative during freeze-substitution.
An alternative protocol for freeze-substitution is described. Araldite/Epon embedding medium (20% in acetone) is first used as a stabilizer (as e.g., OsO(4)) and then as embedding medium. The major components of the Araldite/Epon resin formulation react with proteins and lipids and provide for an excellent preservation and reasonable visualisation of the ultrastructure. The ultrastructural appe...
متن کاملStructure of dictyosomes in Scenedesmus acuminatus observed by the freeze-substitution method.
The ultrastructure of dictyosomes in Scenedesmus acuminatus was studied by the freeze-substitution method. The cisternal membranes of dictyosomes clearly exhibited three-layered structures. Electron-dense particles were visible on or in the proximal cisternal membranes. The upper and lower membranes of the distal cisternae were in close contact with each other at the cisternal centre. The elect...
متن کاملRecent progress in the freeze-etching technique.
The freeze-etching technique must be improved if structures at the molecular size level are to be seen. The limitations of the technique are discussed here together with the progress made in alleviating them. The vitrification of living specimens is limited by the fact that very high freezing rates are needed. The critical freezing rate can be lowered on the one hand by the introduction of anti...
متن کاملRecent progress in the freeze-etching technique
The freeze-etching technique must be improved if structures at the molecular size level are to be seen. The limitations of the technique are discussed here together with the progress made in alleviating them. The vitrification of living specimens is limited by the fact that very high freezing rates are needed. The critical freezing rate can be lowered on the one hand by the introduction of anti...
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ژورنال
عنوان ژورنال: Journal of Histochemistry & Cytochemistry
سال: 1961
ISSN: 0022-1554,1551-5044
DOI: 10.1177/9.3.292